Journal: BioMed Research International
Article Title: Mitochondria Biogenesis and Bioenergetics Gene Profiles in Isogenic Prostate Cells with Different Malignant Phenotypes
doi: 10.1155/2016/1785201
Figure Lengend Snippet: Validation of differential expression of mitochondrial uncoupling protein-2 and SOD2 in RC77N/E and RC77T/E in cell lysates. Normalized total protein lysates from the two cell lines were subjected to Western blot analysis to detect UCP2 (a) and SOD2 (b). All the samples were normalized with respect to GAPDH, which was included as a loading control. The corresponding ImageJ and normalized densitometry comparisons are shown in (c) and (d), respectively.
Article Snippet: Goat polyclonal antibodies against human UCP2 (sc-6526), rabbit polyclonal antibodies against SOD2 (sc-30080), and rabbit polyclonal antibodies against GAPDH (sc-25778) were from Santa Cruz Biotechnology Inc. All prostatectomy tissue samples were collected from patients after informed consent following Institutional Review Board-approved protocols at the Eastern Virginia Medical School.
Techniques: Biomarker Discovery, Quantitative Proteomics, Western Blot, Control